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All experiments described in this study were carried out with the approval of the Penn State University Institutional Animal Care and Use Committee and in accordance with its guidelines

The number of reads matched to each guide in each sample was used to obtain a guide-by-sample count matrix for further analysis

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Gliadel, a polyanhydride-based wafer containing carmustine (BCNU), is designed for optimal drug release, achieving substantial polymer degradation within three weeks of implantation

IV delivery bypasses the digestive system, providing higher absorption and immediate bloodstream availability compared to oral forms