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For anti-C1q or anti-NeuN antibody staining, a DAKO EnVision + System (Agilent Technologies, Santa Clara, CA, USA) was used as the second antibody and the signal was detected using tyramide-enhanced fluorescein isothiocyanate (FITC), as for the TSA method

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[PubMed: 15582288] 39

High levels of glucocorticoids make neurons across the brain more susceptible to oxidative insults via well-described actions of glucocorticoids, including downregulating several antioxidant enzymes and increasing baseline levels of ROS production (ahin and Gml, 2004