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Toxicity evaluations demonstrate an IC50 above 300 g/mL for joint cells and no adverse effects in 28-day in vivo studies

Studies in the U.S

The WT and KO cells at day 8 of spontaneous differentiation were dispersed to single cells using TrypLE Select (Thermo Fisher Scientific, Netherlands), counted, spun down at 500 x g for 5 min at 4 C and resuspended at a concentration of 5 104 cells/50 L per biological replicate in cold ATAC-RSB (10 mM Tris-HCl (Bio-Shop, Canada) pH = 7.4, 3 mM MgCl 2 (Millipore, Germany), 10 mM NaCl (Bio-Shop, Canada), containing 0.1% (v/v) Tween-20 (Roche Diagnostics, Germany), 0.1% (v/v) NP-40 (Biosciences, USA))

For long-term preservation, refrigeration or freezing at 20 C is recommended

In-vitro and in-vivo studies

A.BuehnerG.ChangY.HarperJ