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Michael Brines at The Feinstein Institute for Medical Research, Northwell Health, New York), ARA-290 was specifically engineered to dissociate erythropoietins two major functional activities: Erythropoiesis (red blood cell stimulation driven by EPOR homodimer activation) eliminated in ARA-290 Tissue protection and repair (anti-inflammatory, anti-apoptotic, neuroprotective driven by IRR activation) selectively retained in ARA-290 This elegant dissociation is the core of ARA-290s research significance

2.5.6 Quantification of inflammatory cytokines by ELISA assay in vivo The expression of TNF-, IL-6, IL-17, and EGFR in mice skin tissue was assessed using an Elisa method at a wavelength of 450nm, as instructed by the manufacturer

doi: 10.1111/acel.12232 138 LinC-YC-KTsengC.-K.WuY.-H.LiawC.-C.LinC-YC-KHuangC.-H.et al

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Lysionotin induces ferroptosis to suppress development of colorectal cancer via promoting Nrf2 degradation