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Every brain reacts slightly differently to medications, so it is important to work closely with a healthcare provider to make sure that a medication is the best fit possible

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On day 25, the permeable inserts with monolayers attached were placed into the CellZscope plate (nanoAnalytics GmbH, Germany) for real-time TEER measurements

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Cells were washed twice with CyFACS buffer (PBS with 4% FBS, 0.05% sodium azide), followed by primary antibody staining for 30 mins on ice