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These findings offer valuable insights into the immune evasion strategies employed by Plasmodium in non-adapted hosts and underscore the critical role of extracellular development in the diseases pathogenesis

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Cryoprotective steps in graded sucrose solution (10%, 20%, and 30% sucrose in PB) were performed before the brains were cut on a freeze microtome (Thermo ScientificTM Microm KS 34) in 40 m free floating coronary sections and stored in 0.1 % FA in PB at 4?C protected from light until usage