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Genotype was determined by probing for 24 single nucleotide polymorphisms (SNPs)

Annals Anim Sci

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In combination with other prescription medications, it is helpful in managing chronic illnesses such as heart problems, anaemia, high blood pressure, PCOS, prediabetes, cataracts, arthritis, diabetic retinopathy and infertility

On an experimental day, cells were washed with Hanks Balanced Salt Solution (HBSS, Gibco/ThermoFisher Scientific) and the respective receptors were labeled with 100 nM HaloTag NanoBRET 618 ligand (Promega Corporation, Fitchburg, WI) in phenol red-free Opti-MEM I reduced serum medium (Gibco/ThermoFisher Scientific) (2 h, 37 C, 5% CO 2 )

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