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Nuclear factor erythroid-2 related factor 2 (Nrf2) is an important transcription factor that regulates cellular oxidative stress, which binds to antioxidant response element sites and regulates antioxidant-mediated gene expression and induction ( Early studies have shown that heavy metal Cd promotes the release of iron in biomembrane and this free iron participates in Fenton-type reactions, thus generating ROS ( 0 site of cytochrome b of mitochondrial complex III, leading to the accumulation of semiubiquinone at the q 0 site, generating ROS and causing oxidative stress (Wang et al., 2004)

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50(6): 6779 Novembre E, Frongia G, Lombardi E, et al

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doi:10.1038/273120a0

These limitations highlight the need for standardized protocols, expanded biomarker profiling, and longitudinal human trials in future research