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Add each solvent one by one: PBS Solubility: 7.69 mg/mL (52.62 mM)

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GPX4 shRNA sequences were as follows: shGPX4 1#: GCTACAACGTCAAATTCGA shGPX4 2#: GAGGCAAGACCGAAGTAAA To generate stably transfected cell lines, 293FT cells were cotransfected with the lentivirus packaging vector and shRNA knockdown plasmids