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While the alternative of conducting these experiments on pancreata harvested from THR-123-treated mice would theoretically enable the analysis of time points significantly more extended than those afforded by the limited functional lifespan of slices in vitro, the dissociation of viable cells (especially acinar) from whole pancreatic tissue remains a major technical hurdle in the field 28

Prompted by improved glucose tolerance in AK BSO , we quantified insulin and C-peptide in the plasma of 6-h fasted mice