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3 Future direction Non-pharmacological treatments are still exploratory, with certain limitations and areas requiring further investigation

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adhC and estD displayed an enhanced sensitivity to growth inhibition by exogenous formaldehyde but not other aldehydes or carbonyl compounds such as methylglyoxal ( estD mutant when compared with the adhC single mutant or the adhC-estD double mutant

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Following 24 h incubation, cell viability was measured using the MTT assay