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Both GSS and GCLC are crucial enzymes that are involved in the catalytic rate limiting step and second step reaction, respectively, in the biosynthesis of GSH ( Figure 3 )

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Cell Death Dis 17:224

The plasma sample, along with a spiked internal reference standard (synthetic long-R3-IGF-1 (LR3)), was incubated for 30 minutes

In contrast, RMS13 rhabdomyosarcoma cells that overexpress HRAS, KRAS, or NRAS are resistant to erastin and RAL3 (Schott et al., 2015), which means RAS does affect erastin sensitivity, while the oncogenic RAS pathway is not the sole determinant of ferroptosis sensitivity ( Q 61 L expressing HL-60 cell line, high mobility group box 1 (HMGB1) is an essential regulator of erastin-induced ferroptosis (Ye et al., 2019)