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IDO inhibits a tryptophan sufficiency signal that stimulates mTOR: a novel IDO effector pathway targeted by D-1-methyl-tryptophan

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At harvest, phosphate-buffered saline containing NEM (10 mM) was added and the cells were then kept on ice for 10 min in lysis buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 1% sodium deoxycholate, protease inhibitors, and 10 mM NEM)

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Like probucol, succinobucol had antioxidant activity

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