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For extraction of intracellular metabolites, cells were washed on ice with 1 mL ice-cold 150 mM ammonium acetate (NH 4 AcO, pH 7.3)

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The second main pathway of glycine utilization involves the production of CO 2 and NH 4 + by the reverse reaction of the glycine synthase or glycine cleavage system mentioned above [41,44] (Figure 1)

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