elution reduced glutathione for gst-egfp fusion protein Transglutaminase 2 crosslinks the S-transferase tag, impeding protein–protein interactions of the fused GFP expression in E. coli.
GFP expression in E. coli. (A) Purification workflow of GST fusion Download Scientific Diagram GST Tag Definition & Data GST His purification: A Two step Affinity Purification Protocol Yielding Full length Purified Proteins PMC Research Progress Fusion Tags for Recombinant Protein Production Yuan 2025 Biotechnology and Applied Biochemistry Wiley Online Library Glutathione Resin for Affinity Purification of Proteins In house preparation of hydrogels for batch affinity purification of glutathione S transferase tagged recombinant proteins BMC Biotechnology Springer Nature Link
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