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Cell culture and reagents The human gastric adenocarcinoma cell lines SGC7901 (obtained from the Academy of Military Medical Science, Beijing, China) and the MDR variants, SGC7901/ADR and SGC7901/VCR (established and maintained in our laboratory), and AGS (obtained from the cell bank of Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (Hyclone) and penicillin/streptomycin

This process involves unrestricted lipid peroxidation caused by the generation of reactive oxygen species (ROS) because of an abrupt increase in free Fe 2+ in the cytoplasm, all of which leads to subsequent membrane damage and iron-dependent cell death, now known as ferroptosis

Each vial should be inspected visually before each use for any signs of particulate matter, discoloration, cloudiness, or other changes in appearance

In highly glycolytic tumors, Treg cells take up lactate through monocarboxylate transporter 1

PWL, paw withdrawal

doi: 10.1086/507543 198