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In general, for both the optimization and robust validation of metabolite-derived machine learning models, independent analyses with larger sample sizes of de novo patients across multiple distinct cohorts are needed

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The animals were housed under controlled environmental conditions, including a constant temperature of 22 2 C, a 12-hour light/dark cycle, and 5060% relative humidity

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The diverse functions of metabolic enzymes facilitate the integration of various signals, enabling the precise transduction of external stimuli into cellular fate determinations 30

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