sgOCT4 CRISPRa Controls Vector | Format – SELECT ONE::sgOCT4 Dual sgRNA in pRSL10Ble-mU6-sg1-hU6-sg2-UbiC-Tag RFP-2A-Bleo | Plasmid Both the 43K and 87K

Both the 43K and 87K libraries are designed using the criteria for effective guides described by DeWeirdt et al

as it is the hypervariable region and contains crucial information for studying immune repertoire diversity

Description

Lentivirus expressing luc with BlastR (virus)

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sgOCT4 CRISPRa Controls Vector | Format<span class="variant-dropdown"> – SELECT ONE:</span>:sgOCT4 Dual sgRNA in pRSL10Ble-mU6-sg1-hU6-sg2-UbiC-Tag RFP-2A-Bleo | Plasmid Both the 43K and 87KCellecta's sgOCT4 CRISPRa control constructs are human OCT4targeting sgRNAs in Cellectas standard CRISPRa lentiviral backbones, available either as plasmid DNA or packaged lentiviral particles for direct transduction. SinglesgRNA controls in pRSG16 U6 (sg) UbiC RFP Puro provide a straightforward positive control for CRISPRa activation, while the dualsgRNA pRSL10Ble mU6 sg1 hU6 sg2 UbiC TagRFP 2A Bleo formats offer stronger promoter activation and