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This has been demonstrated in a variety of cancer cell lines, tumor xenografts, spontaneous mouse tumors in transgenic animals and human tumor samples (references in Table 1 )

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Cell viability was assessed using CellTiter-Glo Luminescent Cell Viability Assay (Promega) per manufacturer instructions on a CLARIOstar Plate Reader (BMG Labtech)

However, a more detailed understanding of the expression profile and reliance of these transporters is required

The global sensitivity analysis also confirmed the importance of the redox cycle between SQ . and H 2 Q ( R 4 ), showing that the reverse rate constant, k m4 (SQ . reformation), was the second most sensitive (main interactions) with respect to model OCR and therefore ROS production, suggesting auto-oxidation is an essential part of the doxorubicin redox mechanism

Among these inhibitors, linear polyglycerol sulfate (LPG 20 S 0.94 , degree of sulfation determined via elemental analysis) exhibits optimal inhibitory activity, with an IC 50 value of 67 mg mL -1