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Studies highlight that vitamin B12, along with folate, is needed as a major determinant of one-carbon metabolism, which produces the compound called SAM-e ( S-adenosyl methionine)

For SARS-CoV-2 M pro we used our novel tetrapeptide QS1 fluorogenic substrate, that has a lower k cat /K M value (k cat /K M = 859 s 1 M 1 ) in comparison to FRET substrates 22

Similarly, other markers of Methyl B12 deficiency, Homovanillic acid, Vanillyl Mandelic acid, Quinolinic Acid and Kynurenic acid all increased as glutaric acid increased

It's interesting to note that adding nutrients to KBs doesn't always provide full benefits

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Lu J, Li W, Du X, Ewert DL, West MB, Stewart C, Floyd RA, Kopke RD