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The Z domain, a variant of the B domain of SpA, has been also engineered to create a highly charged Z domain Z basic through the introduction of charged residues at specific positions, which allowed the interaction with negatively charged matrices under physiological conditions (Grslund et al., 2000, Hedhammar et al., 2006)

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All tubes, whether treated with PMA or not, were then centrifuged at 10,000g for 5 min to collect cell pellets, and the supernatants were removed

The results indicated that APE significantly reduced TNF- and IL-6 levels while increasing IL-10 production, suggesting strong anti-inflammatory potential