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Senolytic agents include natural compounds such as quercetin 13 or fisetin 14 , -galactosidase-activated nanoparticles and prodrugs 15,16,17 or compounds that target different vulnerabilities of senescent cells, such as HSP90 inhibitors 18 , cardiac glycosides 19,20 or NMT inhibitors 21

J Lipid Res 50:15091520 Ilyin SE, Belkowski SM, Plata-Salamn CR (2004) Biomarker discovery and validation: technologies and integrative approaches

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Suspended cells were lysed with three freezing-thaw cycles in liquid nitrogen and subsequent ultra-sonication

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