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Western blotting Western blot analysis was performed as described previously [27]

To address sleep disturbances, melatonin is frequently employed

During sustained ERS, PERK phosphorylates eukaryotic initiation factor 2 (eIF2), leading to activating transcription factor 4 (ATF4)-mediated upregulation of the pro-apoptotic transcription factor CHOP (90, 91)

Hydrolyzed 520: Spikes amino acid levels quickly Provides rich leucine-containing peptides Enhances recovery Maximizes muscle-building potential Research: Hydrolyzed whey protein leads to faster amino acid delivery and significantly greater MPS vs intact whey protein

Finally, for recording fatigue levels, the present study adopted BRAF-NRS Question 1 (37), which consists of a single item and is therefore well suited for clinical routine