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NAD+ (nicotinamide adenine dinucleotide) is a naturally occurring molecule found in every cell and plays an essential role in energy metabolism and cellular function

Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma, IPVH00010), blocked for 1 h using 5% BSA in TBS0.5% Tween-20 and stained overnight with primary rabbit-anti-GCLC (Sigma-Aldrich, HPA036359) or affinity-purified rabbit-anti-GGT1 (GGT129, as previously described 63 ) 1:1,000 in BSA 2.5% overnight at 4 C

Periodic boundary conditions and a 2 fs timestep were used

Neuron 104 , 529543.e526 (2019)

Healing rates reveal that small tears achieve a 66% healing rate, while massive tears only reach 27%