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Following incubation, the cells were washed and stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA), 750 ng/mL ionomycin, and 10 g/mL brefeldin A (all from eBioscience) for 4 h at 37 C in a 5% CO 2 environment

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[118] [119] Other subjects [edit] Education [edit] Education follows the three-tier model, which includes primary schools, followed by secondary schools (high schools) and tertiary education at universities or polytechnics