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Chemistry 22(27):93879395

Its important to keep in mind that Glow Blend is not ideal for everyone

GSH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour

Importantly, binding of biotin-CCW16 to GST alone was not detected indicating that in vitro CCW16 bound to RNF4, but not via the proposed cysteine residues C132 and C135