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To differentiate HL-60 to a neutrophil-like state, cells were transferred to differentiation medium (RPMI with 1.3% DMSO, 5 mM glutamine, 25 mM HEPES, 9% FBS and 1% penicillin/streptomycin) for 6 days, with medium change on day 3

Kruman II, Culmsee C, Chan SL, et al

In vitro studies, although useful for clarifying molecular pathways as NF-B suppression, antioxidant enzyme overexpression, sometimes employ elevated, non-physiological concentrations of extracts and may fail to correctly simulate the complex context of the human eye

Plasmacytoid dendritic cell activation is dependent on coordinated expression of distinct amino acid transporters