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Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

The first enzyme of the urea cycle is CPS1, which catalyzes the conversion of ammonia, bicarbonate and adenosine triphosphate to carbamoylphosphate, the initial rate-limiting step of the urea cycle [1]

Release of BPC-157 from the bacterial surface or from isolated fusion proteins by trypsinization was demonstrated with anti-BPC-157 antibodies or by mass spectrometry

Lele Wang, [email protected] These authors have contributed equally to this work and share first authorship These authors have contributed equally to this work Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

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For microinjection, 2-day-post-fertilization larvae ( p22 / or p22 +/+ ) were anesthetized with 0.2 mg ml 1 Tricaine (Sigma), and 3 nl of A