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After that, 50 L 20% HClO 4 was added to the 450 L of pretreated tissue, blood samples, or thawed plasma samples, and the proteins were precipitated by centrifugation at 19,090 g for 5 min at 4C

The secondary plot for the first k obs values from the second phase yielded a rate contant at GSSG saturation around 10 s 1 for all three PfGrx variants (Fig

ATP should then be rapidly used up and have a low measured concentration

The significant increase in GST activity further supports the theory that this could be a response directly linked to microplastic exposure

Am J Transplant (2006) 6:90312

Merck Millipore, Burlington, MA, USA) staining was carried out according to the manufacturers instructions