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GTP serves as the precursor for BH4 synthesis, a process requiring three enzymatic steps catalyzed by GCH1, 6-propylthio-tetrahydropterin synthase (PTS), and spirodactyl reductase (SPR), with GCH1 acting as the rate-limiting enzyme [64]

The improvement was attributed to curcumins ability to modulate gut microbiota composition and enhance SCFA production, which positively influenced gut barrier integrity and reduced systemic inflammation

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DAG expression: high-throughput gene expression analysis of real-time PCR data using standard curves for relative quantification