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Chromatographic conditions: ExionLC Ultra Performance Liquid Chromatography (UPLC) system (SCIEX Corp.), including liquid phase pump, integrated degassing device, autosampler, column temperature chamber, etc

Relevant studies indicate that TMAO levels are abnormally high in hypercholesterolemia patients, suggesting a close link between TMAO and the pathogenesis of hypercholesterolemia (Dehghan et al., 2020)

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In addition to analyzing the 12 metabolites as individual molecules, we calculated pairwise correlations to examine potential relationships among the 12 compounds (Supplementary Fig