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Experimental design Five equal groups of eight cycled female rats each were randomly assigned to the following groups ( Fig

Media was removed after 24 h and cells washed with PBS before addition of 90 l PBS containing 0.49 mM MgCl 2 6H 2 O, 0.9 mM CaCl 2 2H 2 O, 0.1% BSA and 0.11 M Nano-Glo Substrate