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To determine whether these residues mediate direct interaction between BubR1 and Mad2, we purified recombinant His-tagged Mad2 L13A, a mutant that keeps Mad2 in the closed conformation and GST-tagged wild type or W22A/E23A BubR1 (residues 1-220), and performed GST pull-down assay

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The authors acknowledge the Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, for providing the necessary research facilities