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After resuspending in PBS, the suspension was extruded through 800 nm and 200 nm porous polycarbonate filters for 11 times to prepare RAW 264.7 cell-membrane nanovesicles (RCM)

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Another thirty units (Speywood) botulinum toxin type A (Dysport) was injected in two locations into the external nasal trigger zone taking care to remain superficial and not to inject levator labii superiorus

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Similarly, the catalytic activity of other nanozymes must also be investigated when dispersed in relevant buffers, to achieve any biological application