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Gene expression analysis For real-time PCR analysis, total RNA was extracted with Trizol (Invitrogen) and ethanol precipitation, and subsequently treated with Amplification Grade DNAse I (Invitrogen)

doi:10.3390/nu15030733

For example, gestational protein restriction in mice decreased H3K4me3 and H4K20me3 while it increased H3K9me3 and H3K27me3 levels on the Igf2 (Insulin-like growth factor-II) locus, resulting in its repression in fetal liver (Strakovsky et al., 2011)