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The resulting cDNA was amplified using 2 SYBR mix (Qiagen) and 1 M of each primer in a StepOne Plus Thermocycler (Applied Biosystems) in Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR)

This study showed that cytosolic nuclear DNA did not induce proinflammatory responses in HUVECs, although STING can detect cytosolic nuclear DNA [9]

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It's a state with a proud hunting tradition, and its robust white-tail deer population makes it a premier destination