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This pathway-level enrichment is directly corroborated by our functional co-expression network, which highlights the up-regulation of key metabolic nodes such as AmPCT2 (phenylalanine biosynthesis) and AmAGBE3 (starch and sucrose metabolism)

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The urea cycle, discovered by Hans Krebs and Kurt Henseleit 546 , converts toxic ammonium (obligatory product of amino acids catabolism) to urea

Additionally, SLC7A11 influences the immune response to therapy by regulating the redox balance and immune cell infiltration (such as inhibiting CD8 + T cell activity) in the tumor microenvironment, and its expression is subject to multi-level regulation by epigenetic modifications (such as m6A, ubiquitination) and transcription factors (NRF2, ATF4), offering diverse targets for the development of small molecule inhibitors, metabolic interventions (glucose deprivation), or combination therapies (such as with immune checkpoint inhibitors)

However, further research on appropriate dosing, clinical benefits, and adverse effects is needed through large prospective trials involving patients with MASLD