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Details of cloning, mutagenesis, transfection of K562 cells and luciferase measurements are described in the Online Supplementary Methods .23 Quantitative (q)RT-PCR to quantify GATA1, GATA2, NBEAL2, and ITGB3 expression Total ribonucleic acid (RNA) from K562 cells was extracted with TRIzol (Invitrogen)

[DOI] [PMC free article] [PubMed] [Google Scholar] Stoica, R

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It's a well-established standard based on the calculated effectiveness of the benzyl alcohol preservative under ideal conditions

doi: 10.1016/j.pharmthera.2022.108238 15 CaiT