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The frozen-thawed sperm viability was analyzed by eosin-nigrosin staining (55, 58)

Together, these findings indicate that while strong oxidants, such as H 2 O 2 , favor irreversible thiol overoxidation and suppress disulfide chemistry, reactive nitrogen species provide a physiologically relevant pathway for disulphide cross-linking of TTR

It does this by scavenging reactive oxygen species (ROS), which helps protect mitochondrial components such as proteins, lipids, and DNA from oxidative damage, thereby preserving mitochondrial function

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