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Rabbit polyclonal anti-caspase-3 (WB, 9662) used for human, mouse and zebrafish samples, and rabbit monoclonal anti-GSDMD (WB, 97558) antibodies were from Cell Signaling Technology (Danvers, USA), rabbit monoclonal anti-GSDME (WB/IP, ab215191) used for human, mouse and zebrafish samples, and rabbit monoclonal anti-granzyme B (WB, ab255598) antibodies were from Abcam (Cambridge, USA), rabbit polyclonal anti-GSDME (IF, A7432) was from ABclonal (Wuhan, China), mouse monoclonal anti-caspase-3 (WB/IP, sc-271759) and mouse monoclonal anti-granzyme B (WB/IP, sc-8022) were from Santa Cruz Biotechnology (Dallas, USA), rabbit monoclonal anti-GAPDH (AB0037) was from Abways Technology (Shanghai, China)

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The supernatant was stored at 4 C and used for CAT, POD, SOD and GR assays

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